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Submitted
Abstract
Identification of key genes related to efferocytosis in cystitis glandularis using single-cell transcriptome and experimental validation
Non-Moderated Poster Abstract
Basic Research
Oncology: Bladder and UTUC
Author's Information
1
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China
Yunzhi Lin 389842855@qq.com The first affliated hospital of Fujian medical university Urology fuzhou China *
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Abstract Content
Cystitis glandularis (CG) is a proliferative disease of bladder caused by inflammatory stimuli and no definitive treatment have been identified. Efferocytosis has anti-inflammatory effect in human body, but the role of efferocytosis in CG has not been clarified. Therefore, this study intended to identify key genes related to efferocytosis in CG.
The single-cell dataset GSE225190 was gained from public database. After single-cell data was processed, downscaled and clustered, cell cluster which had the highest proportion and exhibited difference between CG and control groups was identified as key cell cluster. Differential expressed genes (DEGs) in key cell cluster, along with 103 efferocytosis related genes were intersected to acquire candidate genes. Then a protein-protein interaction (PPI) network was developed and key genes were selected via the network. According to the expression of key genes, pseudotime analysis was performed for key cell cluster, and in-depth discussions were conducted on key genes including chromosome and subcellular localization, transcription factors (TFs) prediction, gene gene interaction (GGI) network, regulatory and drug prediction networks construction. Finally, expression of key genes were validated via reverse transcription-quantitative PCR (RT-qPCR).
Epithelial cells were identified as key cells, which had strong interaction with other cells in CG group. Totally 6 key genes (ANXA1, ANXA2, LGALS3, CALR, CLU and GAPDH) which expressed highly in CG group were identified. According to expression of key genes, epithelial cells were clustered into 3 cell types, among them key genes were mainly expressed in stem cell like epithelial cells, which showed a later stage of differentiation. Localization analyses indicated 6 key genes were located at chromosome 9, 15, 14, 19, 8 and 12, respectively. ANXA1, CALR, GAPDH, LGALS3 were located at cytosol and other 2 key genes were in nucleus. TF POLR2A regulated 6 key genes simultaneously and drug therapeutic glucocorticoid targeted to CALR and ANXA1 simultaneously. Finally, expression of ANXA1, ANXA2, LGALS3 and CALR were validated in clinical samples.
We identified 6 key genes (ANXA1, ANXA2, LGALS3, CALR, CLU and GAPDH) related to efferocytosis in CG, providing valuable insights for CG prevention and treatment.
Cystitis glandularis, Efferocytosis, Single-cell RNA sequencing, Key genes
 
 
 
 
 
 
 
 
 
 
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Presentation Details