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Non-Moderated Poster Abstract

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Abstract

Abstract Title

KLF2 as a Tumor Suppressor in clear cell renal cell carcinoma: the induction of the DNA Damage in ccRCC.

Presentation Type

Non-Moderated Poster Abstract

Manuscript Type

Basic Research

Abstract Category *

Oncology: Kidney (non-UTUC)

Author's Information

Number of Authors (including submitting/presenting author) *

No more than 10 authors can be listed (as per the Good Publication Practice (GPP) Guidelines).
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Co-author 1

Hong-Cheng Wang phxforwork@gmail.com Chang Gung Memorial Hospital at Linkou Division of Urology, Department of Surgery Taoyuan 33305, Taiwan, R.O.C Taiwan *

Co-author 2

Tzu-hsuan Chang sateen1017@gmail.com Chang Gung Memorial Hospital at Linkou Division of Urology, Department of Surgery Taoyuan 33305, Taiwan, R.O.C Taiwan -

Co-author 3

Tzu-Kai Wang ralph333@cgmh.org.tw Chang Gung Memorial Hospital at Linkou Division of Urology, Department of Surgery Taoyuan 33305, Taiwan, R.O.C Taiwan -

Co-author 4

Cheng-keng Chuang chuang89@cgmh.org.tw Chang Gung Memorial Hospital at Linkou Division of Urology, Department of Surgery Taoyuan 33305, Taiwan, R.O.C Taiwan -

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Abstract Content

Introduction

In kidney cancer, renal cell carcinoma(RCC) is the most common type, accounting for approximately 90%. Among RCC cases, clear cell renal cell carcinoma(ccRCC) makes up the majority. Many studies have shown the impact of Kruppel-like factor 2(KLF2) on RCC. The aim of this study is to observe how KLF2 induces cell senescence in RCC.

Materials and Methods

KLF2 expression was evaluated in ccRCC tissues using immunohistochemistry (IHC) and quantitative PCR. ccRCC cell lines (786-O, UOK171) were subjected to KLF2 overexpression, and cell viability was measured using the CCK-8 assay. Phase contrast and GFP fluorescence images of 786O and UOK171 cells transfected with control (GFP) or KLF2 overexpression (KLF2-GFP) constructs were performed.

Results

KLF2 expression was downregulated in ccRCC tissues compared to normal renal tissues. KLF2 overexpression induced cell Senescence and DNA damage in ccRCC cell lines. In the 786O cell line, the expression level of DNA damage marker γH2AX shows a significant difference between the control group and the KLF2 overexpression group. (p<0.0001) In the UOK171 cell line, the expression level of γH2AX also shows a significant difference between the control group and the KLF2 overexpression group. (p=0.0051)

Conclusions

In ccRCC cells, KLF2 is an important regulator of cellular senescence. This result indicates the potential of KLF2 to inhibit the tumor growth of ccRCC, making it a promising target for future ccRCC therapies.

Keywords

ccRCC, KLF2

Figure 1

https://storage.unitedwebnetwork.com/files/1237/078e47b7fc3048f359b689f4dadf6b08.jpg

Figure 1 Caption

Immunofluorescence staining of 786O cells and UOK171 cells for DNA damage marker γH2AX (red) in control and KLF2-overexpressing cells.

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