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Abstract
Study on the mechanism of erectile dysfunction in D-galactose induced aging rats: the construction of aging model
Moderated Poster Abstract
Basic Research
Andrology: Sexual and Erectile Dysfunction
Author's Information
6
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China
Taotao Sun suntt94@163.com the First Affiliated Hospital of Zhengzhou University Department of Urology zhengzhou China *
Penghui Yuan yuanph2018@126.com the First Affiliated Hospital of Zhengzhou University Department of Urology zhengzhou China
Yipiao Liu zzlyp2019@163.com the First Affiliated Hospital of Zhengzhou University Department of Hepatopancreatobiliary Surgery zhengzhou China
Zhankui Jia jiazhankui@126.com the First Affiliated Hospital of Zhengzhou University Department of Urology zhengzhou China
Jinjian Yang yangjinjian2011@126.com the First Affiliated Hospital of Zhengzhou University Department of Urology zhengzhou China
Jihong Liu jhliu@tjh.tjmu.edu.cn Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Department of Urology Wuhan China
 
 
 
 
 
 
 
 
 
 
Abstract Content
With the aging of the population, more and more patients are suffering from aging-related erectile dysfunction (ED), and its related clinical and basic research has gradually attracted attention. However, the traditional natural aging model has a long breeding cycle and large differences among individuals. D-galactose can simulate the natural aging process and is gradually used to construct rodent aging models. Therefore, we aimed to establish an ideal and reliable model of aging-related ED in vivo and in vitro using D-galactose.
The corpus cavernosum smooth muscle cells (CCSMCs) from SD rats were primarily extracted and cultured. CCSMCs and HUVEC were respectively set as D-galactose concentration gradient groups: 0g/L, 5g/L, 10g/L and 20g/L. Cell proliferation potential, cell cycle, oxidative stress and DNA damage in each group were detected. In addition, CCSMCs were cultured with HUVEC medium, and the functions of endothelial and smooth muscle cells were also detected. In animal experiments, D-galactose was injected intraperitoneally to construct an aging model of rats. The erectile function was measured, then corpus cavernosum samples were collected for detection.
1. Cell experiments: Under the influence of D-galactose, both CCSMC and HUVEC showed increased β-galactosidase activity, decreased cell proliferation ability, cell cycle arrest, increased level of oxidative stress and DNA damage. And the above changes showed concentration dependence of D-galactose. In the two-cell culture system, the results showed that the expression of eNOS and NO in HUVEC decreased, and the content of cGMP in CCSMC also decreased, suggesting that the function of endothelial and smooth muscle cells was impaired. 2. Animal experiments: Compared with the normal rats, the rats injected with D-galactose showed an aging body and impaired erectile function. The oxidative stress level in the corpus cavernosum increased, while the content of smooth muscle and endothelium decreased.
We found that D-galactose could damage the function of smooth muscle and endothelial cells by inducing oxidative stress, and successfully established an aging-related ED model. These results provide a reliable in vitro and in vivo model for studying the mechanisms of age-related ED.
erectile dysfunction; aging; D-galactose; oxidative stress
 
 
 
 
 
 
 
 
 
 
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Presentation Details