Back
Non-Moderated Poster Abstract
Eposter Presentation
 
Accept format: PDF. The file size should not be more than 5MB
 
Accept format: PNG/JPG/WEBP. The file size should not be more than 2MB
 
Withdrawn
Abstract
Pro-Regenerative Effect of iMSC-EV on Urethral Epithelial Cells: An In Vitro Evaluation of Proliferation and Migration
Non-Moderated Poster Abstract
Basic Research
Novel Advances: Other Urology Translational Studies
Author's Information
6
No more than 10 authors can be listed (as per the Good Publication Practice (GPP) Guidelines).
Please ensure the authors are listed in the right order.
Japan
Kazuki Yanagida k.yanagida.sr@juntendo.ac.jp Juntendo University Graduate School of Medicine Department of Molecular and Cellular Therapeutics Tokyo Japan *
Daisuke Watanabe da-watanabe@juntendo.ac.jp Juntendo University Graduate School of Medicine Department of Molecular and Cellular Therapeutics Tokyo Japan -
Tsuyoshi Kawaharada t.kawaharada.gp@juntendo.ac.jp Juntendo University Graduate School of Medicine Department of Molecular and Cellular Therapeutics Tokyo Japan -
Akio Mizushima akiom@juntendo.ac.jp Juntendo University Graduate School of Medicine Department of Molecular and Cellular Therapeutics Tokyo Japan -
Masayuki Shinchi shinchimasayuki@yahoo.co.jp National Defense Medical College Department of Urology Tokorozawa Japan -
Akio Horiguchi asukamaru513@gmail.com National Defense Medical College Hospital Center for Trauma, Burn and Tactical medicine Tokorozawa Japan -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
Abstract Content
Urethral stricture is a challenging condition characterized by impaired epithelial regeneration and fibrotic remodeling. Recently, extracellular vesicles (EVs) derived from induced pluripotent stem cell-derived mesenchymal stem cells (iMSC-EVs) have shown promise in regenerative medicine. In this study, we evaluated the in vitro effects of iMSC-EVs (UREVEX) on urethral epithelial cell proliferation and migration.
Urethral epithelial cells were cultured under two conditions: (1) control with PBS treatment and (2) treatment with iMSC-EVs (UREVEX). Cell proliferation was assessed by calculating proliferation rates using time-lapse imaging with the CM20 system. Migration capacity was evaluated via a scratch wound healing assay, also analyzed using CM20-based image analysis.
Compared to the PBS control, cells treated with iMSC-EVs (UREVEX) demonstrated significantly increased proliferation rates and accelerated wound closure in the scratch assay. These findings suggest that iMSC-EVs promote both cell proliferation and migration in urethral epithelial cells.
Extracellular vesicles derived from mesenchymal stem cells differentiated from induced pluripotent stem cells significantly enhanced urethral epithelial cell proliferation and migration in vitro. These findings suggest their potential as a novel pro-regenerative therapeutic approach to support epithelial repair and reduce the risk of stricture formation following urethral injury.
extracellular vesicles, iPSC, iMSC, urethral injury, urethral stricture
 
 
 
 
 
 
 
 
 
 
1448
 
Presentation Details
 
 
 
0