Integrated Chip Provide Rapid Identification of Uropathogen

Chia-Hung LiuTaiwan Speaker Integrated Chip Provide Rapid Identification of UropathogenThe present study proposes a unique approach for the quick and cost-effective detection of Escherichia coli (E. coli) in urine samples using disposable gold electrode sensing chips. Spectrophotometry and plate counting demonstrated a significant linear correlation coefficient of 0.98986 between absorbance values and E. coli concentration. The electrochemical detection approach used modified electrodes to increase sensitivity. The method used to address cyclic voltammetry (CV) was not effective in depicting the peaks involved in breaking down the bacterial cell wall barrier using a permeabilizer. Square wave voltammetry (SWV) was used to identify changes in the p-nitrophenyl-β-D-galactopyranoside (p-APG) oxidation peak, which served as evidence of the successful integration and hydrolysis of β-galactosidase (β-gal). Both modified and unmodified gold electrodes showed an inversely proportional oxidation peak at 500 mV for p-PAG in E. Coli experiments employing SWV, with the modified electrodes showing greater sensitivity. During clinical testing, the proposed biosensor successfully detected urine bacterial counts with a sensitivity of about 75 colony-forming units per millilitre (cfu/ml), despite difficulties in detecting extremely low E. coli concentrations. The use of electrochemical techniques resulted in a significant decrease in experimental time, allowing real-time analysis for useful applications in the detection of bacteria in urine.